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Catalog Number | orb20327 |
---|---|
Category | Antibodies |
Description | Goat polyclonal to IREB2 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human, Mouse, Rat |
Dilution range | ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 3-6µg/ml |
Conjugation | Unconjugated |
MW | 105 |
Target | IREB2 / IRP2 |
Entrez | 3658 |
Protein Sequence | SIHYEGSEYKLSHGS |
RRID | AB_10749789 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti IREB2 antibody, anti iron-responsive element Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 110kDa band observed in Human Liver lysates (calculated MW of 105kDa according to NP_004127.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Human Liver lysate using IREB2 antibody
Immunohistochemical staining of Human Kidney using IREB2 antibody