IREB2/IRP2 Antibody

SKU: orb20327

Description

Goat polyclonal to IREB2

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 3-6µg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 110kDa band observed in Human Liver lysates (calculated MW of 105kDa according to NP_004127.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: IREB2 / IRP2
• Protein Sequence: SIHYEGSEYKLSHGS
• Molecular Weight: 105
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti IREB2 antibody, anti iron-responsive element binding protein 2 antibody, anti ACO3 antibody, anti FLJ23381 antibody, anti IRP2 antibody, anti IRP2AD antibody, anti iron regulatory protein 2 antibody

Western blot analysis of Human Liver lysate using IREB2 antibody

Immunohistochemical staining of Human Kidney using IREB2 antibody

Quick Database Links

Gene Symbol

IREB2 / IRP2

NCBI Gene ID

3658