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Catalog Number | orb19859 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to IGFBP6 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Human |
Dilution range | ELISA: 1:32000, WB: 0.5-1.5 μg/ml, IHC-P: 5 μg/ml |
Conjugation | Unconjugated |
MW | 25.3 |
Target | IGFBP6 |
Entrez | 3489 |
Protein Sequence | RPQDVNRRDQQRNP |
RRID | AB_10748554 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti IGFBP6 antibody, anti insulin-like growth fac Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 25kDa band observed in Human Adrenal Gland lysates (calculated MW of 25.3kDa according to NP_002169.1). In transfected HEK293 transiently expressing IGFBP6 a band of approx. 29kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Human Adrenal Gland lysate using IGFBP6 antibody
Western blot analysis of HEK293 lysate using IGFBP6 antibody
orb19859 (5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.