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Catalog Number | orb19656 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to IGFBP3 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:64000, WB: 0.02-0.06 μg/ml |
Conjugation | Unconjugated |
MW | 31.7; 32.2 |
Target | IGFBP3 |
Entrez | 3486 |
Protein Sequence | RYKVDYESQSTDTQN |
RRID | AB_10753579 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti IGFBP3 antibody, anti insulin-like growth fac Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 28kDa band observed in Human Breast cancer lysates (calculated MW of 31.7kDa according to NP_001013416.1 and 32.2kDa according to NP_000589.2). Recommended concentration: 0.02-0.06 μg/ml. An additional band of 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot. |
Expiration Date | 12 months from date of receipt. |
orb19656 (0.02ug/ml) staining of Human Breast cancer lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.