IGFBP3 Antibody

SKU: orb19656

Description

Goat polyclonal antibody to IGFBP3

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:64000, WB: 0.02-0.06 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 28kDa band observed in Human Breast cancer lysates (calculated MW of 31.7kDa according to NP_001013416.1 and 32.2kDa according to NP_000589.2). Recommended concentration: 0.02-0.06 μg/ml. An additional band of 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: IGFBP3
• Molecular Weight: 31.7; 32.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti IGFBP3 antibody, anti insulin-like growth factor binding protein 3 antibody, anti tcag7.703 antibody, anti BP-53 antibody, anti IBP3 antibody, anti IGF-binding protein 3 antibody, anti acid stable subunit of the 140 K IGF complex antibody, anti binding protein 29 antibody, anti binding protein 53 antibody, anti growth hormone-dependent binding protein antibody

orb19656 (0.02ug/ml) staining of Human Breast cancer lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

IGFBP3

NCBI Gene ID

3486