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Goat anti-IGFBP3 Antibody

Catalog Number: orb19656

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb19656
CategoryAntibodies
DescriptionGoat polyclonal antibody to IGFBP3
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, WB
ReactivityCanine, Human, Mouse, Rat
Dilution rangeELISA: 1:64000, WB: 0.02-0.06 μg/ml
ConjugationUnconjugated
MW31.7; 32.2
TargetIGFBP3
Entrez3486
Protein SequenceRYKVDYESQSTDTQN
RRIDAB_10753579
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti IGFBP3 antibody, anti insulin-like growth fac
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 28kDa band observed in Human Breast cancer lysates (calculated MW of 31.7kDa according to NP_001013416.1 and 32.2kDa according to NP_000589.2). Recommended concentration: 0.02-0.06 μg/ml. An additional band of 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Expiration Date12 months from date of receipt.
Goat anti-IGFBP3 Antibody

orb19656 (0.02ug/ml) staining of Human Breast cancer lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.