ICSBP1/IRF8 Antibody

SKU: orb18409

Description

Goat polyclonal antibody to IRF8

Research Area

Autophagic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:8000, WB: 0.5-2 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 45-50kDa band seen in K562 lysate (Predicted MW of 47kDa according to NP_002154). Recommended for use at 0.5-2 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ICSBP1 / IRF8
• Protein Sequence: QRSFFRENQQITV
• Molecular Weight: 48.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti IRF8 antibody, anti ICSBP1 antibody, anti interferon consensus sequence binding protein 1 antibody, anti IRF-8 antibody, anti H-ICSBP antibody, anti interferon regulatory factor 8 antibody

orb18409 staining (1ug/ml) of K562 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18409 (5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

ICSBP1 / IRF8

NCBI Gene ID

3394