HTATSF1 Antibody

SKU: orb19565

Description

Goat polyclonal antibody to HTATSF1

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:16000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 140kDa band observed in lysates of cell line Daudi (calculated MW of 85.9kDa according to NP_055315.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhou and Sharp, Science. 1996 Oct 25;274(5287):605-10.; PMID: 8849451). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: HTATSF1
• Protein Sequence: QELYGDGKDGDTQTD
• Molecular Weight: 85.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti HTATSF1 antibody, anti HIV-1 Tat specific factor 1 antibody, anti RP1-196E23.2 antibody, anti TAT-SF1 antibody, anti dJ196E23.2 antibody, anti HIV TAT specific factor 1 antibody, anti cofactor required for Tat activation of HIV-1 transcription antibody

orb19565 (1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

HTATSF1

NCBI Gene ID

27336