Fumarase/FH Antibody

SKU: orb19547

Description

Goat polyclonal antibody to FH

Research Area

Cancer, DNA Damage, Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:64000, WB: 0.01-0.03 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 48kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Fumarase / FH
• Protein Sequence: HPNDHVNKSQSSND
• Molecular Weight: 54.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti fumarate hydratase antibody, anti HLRCC antibody, anti LRCC antibody, anti MCL antibody, anti MCUL1 antibody, anti fumarase antibody, anti leiomyomatosis and renal cell cancer antibody, anti multiple hereditary cutaneous leiomyomata antibody

Western blot analysis of Human Kidney lysate using FH antibody

Immunohistochemical staining of Human Skeletal Muscle using FH antibody

Immunohistochemical staining of Human Heart using FH antibody

Quick Database Links

Gene Symbol

Fumarase / FH

NCBI Gene ID

2271