FTL Antibody

SKU: orb20089

Description

Goat polyclonal antibody to Ferritin

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.3-1.0 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: In paraffin embedded Human Brain Cortex shows cytoplasm staining of pyramydal neurons. Data obtained from a previous batch (different goat). Recommended concentration, 3-6µg/ml.WB: Approx. 20kDa band observed in Human Brain (Cerebellum), Liver and Placenta lysates (calculated MW of 20.0kDa according to NP_000137.2). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: FTL
• Molecular Weight: 20
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti-Cell proliferation-inducing gene 15 protein antibody, anti- Ferritin H subunit antibody, anti- Ferritin heavy chain antibody, anti- Ferritin heavy polypeptide 1 antibody, anti- Ferritin L subunit antibody, anti- Ferritin light polypeptide antibody, anti- Ferritin, heavy polypeptide antibody, anti- FRIH_human antibody, anti- FTH antibody, anti- FTH1 antibody, anti- FTL antibody

Western blot analysis of human placenta lysate using FTL antibody

Western blot analysis of Human Cerebellum (A), Liver (B), Placenta (C), Mouse Brain (D) and Rat Brain (E) lysates using Ferritin antibody

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of using Ferritin antibody

Immunohistochemical staining of Human Brain using Ferritin antibody

Quick Database Links

Gene Symbol

FTL

NCBI Gene ID

2512

References

Ferreira, Gabriela Bomfim et al. Differential protein pathways in 1,25-dihydroxyvitamin d(3) and dexamethasone modulated tolerogenic human dendritic cells J Proteome Res, 11, 941-971 (2012)

PubMed: 22103328