FOXD1/FREAC4/FKHL8 Antibody

SKU: orb18553

Description

Goat polyclonal antibody to FOXD1

Images & Validation

• Tested Applications: ELISA, IF
• Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000. Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10µg/ml.
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: FOXD1 / FREAC4 / FKHL8
• Protein Sequence: SSVENFTARISNC
• Molecular Weight: 46.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti FOXD1 antibody, anti forkhead box D1 antibody, anti FKHL8 antibody, anti FREAC4 antibody, anti forkhead (Drosophila)-like 8 antibody, anti forkhead-related activator 4 antibody, anti Forkhead, drosophila, homolog-like 8 antibody, anti forkhead-like 8 antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

FOXD1 / FREAC4 / FKHL8

NCBI Gene ID

2297