Goat anti-FOXB1 / FKH5 Antibody

• Catalog Number: orb18574
• Category: Antibodies
• Description: Goat polyclonal antibody to FOXB1
• Target: FOXB1 / FKH5
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: TSPASALHSVAVH
• RRID: AB_10755563
• MW: 35
• Tested applications: ELISA, ICC, IF, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 0.1-0.3ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave no signal but low background in Human Brain extracts at up to 1 μg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysatesExperiment Notes: Immunocytochemistry: Anonymous customer found nuclear staining at lumbar levels of the Mouse Spinal Cord at embryonic day 12.5. Recommended concentration 0.1-0.3ug/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti FOXB1 antibody, anti forkhead box B1 antibody
• Note: For research use only
• Entrez: 27023

Western blot analysis of HepG2 nuclear lysate using FOXB1 antibody