FHL3/SLIM2 Antibody

SKU: orb20190

Description

Goat polyclonal to FHL3

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:4000, WB: 1-3 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 30kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 31.2kDa according to NP_004459.2). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: FHL3 / SLIM2
• Protein Sequence: DQVLCQGCSQAGP
• Molecular Weight: 31.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti FHL3 antibody, anti SLIM2 antibody, anti four and a half LIM domains 3 antibody, anti LIM-only protein FHL3 antibody, anti MGC19547 antibody, anti MGC23614 antibody, anti MGC8696 antibody

orb20190 (1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

FHL3 / SLIM2

NCBI Gene ID

2275