FACT/SUPT16H Antibody

SKU: orb19521

Description

Goat polyclonal antibody to SUPT16H

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:8000, WB: 0.1-0.3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 150kDa band observed in lysates of cell line HeLa (calculated MW of 120kDa according to NP_009123.1). Recommended concentration: 0.1-0.3 μg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: FACT / SUPT16H
• Protein Sequence: DLTNKEGKKPEEK
• Molecular Weight: 120
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SUPT16H antibody, anti FACT antibody, anti suppressor of Ty 16 homolog (S. cerevisiae) antibody, anti HGNC:11465 antibody, anti CDC68 antibody, anti FACTP140 antibody, anti FLJ10857 antibody, anti FLJ14010 antibody, anti SPT16/CDC68 antibody, anti chromatin-specific transcription elongation factor large subunit antibody, anti FLJ34357 antibody, anti facilitates chromatin remodeling 140 kDa subunit antibody

orb19521 (0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Kidney using SUPT16H antibody

Quick Database Links

Gene Symbol

FACT / SUPT16H

NCBI Gene ID

11198