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Catalog Number | orb20429 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to EYA1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:16000, WB: 1-3 μg/ml |
Conjugation | Unconjugated |
MW | 61.2; 64.6; 60.7 |
Target | EYA1 |
Entrez | 2138 |
Protein Sequence | TDPTAEYSTIHSP |
RRID | AB_10751288 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti EYA1 antibody, anti eyes absent homolog 1 (Dr Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 55kDa band observed in lysates of cell lline HEK293 (calculated MW of 60.7kDa according to NP_742056.1). Recommended concentration: 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb20429 (2ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.