Goat anti-EXO1 Antibody

• Catalog Number: orb19738
• Category: Antibodies
• Description: Goat polyclonal antibody to EXO1
• Target: EXO1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: HRNYSPRPESGT
• RRID: AB_10753581
• MW: 89.1; 94.0
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:64000, WB: 0.2-0.5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 90kDa band observed in lysates in cell lines HEK293 and A549 (calculated MW of 89.1kDa according to NP_003677.3). Recommended concentration: 0.2-0.5 μg/ml. An additional band of unknown identity was also consistently observed at 38kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti EXO1 antibody, anti exonuclease 1 antibody, a
• Note: For research use only
• Entrez: 9156

orb19738 (0.2ug/ml) staining of A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.