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Catalog Number | orb19994 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to ERCC1 |
Target | ERCC1 |
Clonality | Polyclonal |
Species/Host | Goat |
Conjugation | Unconjugated |
Reactivity | Human |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Protein Sequence | DPGKDKEGVPQPS |
RRID | AB_10755874 |
MW | 35.6; 32.6 |
Tested applications | ELISA, WB |
Dilution range | ELISA: 1:64000, WB: 0.3-1 μg/ml |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 38kDa band observed in lysates of cell lines A431 and Kelly (calculated MW of 35.6kDa according to NP_973730.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 μg/ml. |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti ERCC1 antibody, anti excision repair cross-co Read more... |
Note | For research use only |
Entrez | 2067 |
Expiration Date | 12 months from date of receipt. |
orb19994 (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA, IF | |
Bovine, Canine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |