Goat anti-ELF3 / ERT/ ESX Antibody

• Catalog Number: orb18399
• Category: Antibodies
• Description: Goat polyclonal antibody to ELF3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Dilution range: ELISA: 1:32000, WB: 2-4 μg/ml
• Conjugation: Unconjugated
• MW: 41.5
• Target: ELF3 / ERT/ ESX
• Entrez: 1999
• Protein Sequence: SGWKEEEVLQSRN
• RRID: AB_10754451
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ELF3 antibody, anti ERT antibody, anti E74-li
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 48 kDa band observed in NCI-H460 lysate (calculated MW of 41.5kDa according to NP_004424.2). In transfected HEK293 transiently expressing ELF3 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 2-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18399 staining (3ug/ml) of NCI-H460 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

HEK293 overexpressing ELF3 (RC200631) and probed with orb18399 (mock transfection in first lane), tested by Origene.