Goat anti-Duffy / FY / DARC Antibody

SKU: orb19093

Description

Goat polyclonal antibody to DARC

Research Area

Cardiovascular Disease, Epigenetics, GPCR, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded human kidney, stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. This antibody has also been successfully used on HWB: Approx 35kDa band observed in human kidney and human liver lysates (calculated MW of 35.6kDa according to NP_002027.2). Recommended concentration: 0.1-0.3 μg/ml.Experiment Notes: FACS: A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Duffy / FY / DARC
• Molecular Weight: 35.7; 35.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti FY antibody, anti DARC antibody, anti HGNC:4035 antibody, anti Duffy blood group antibody, anti CCBP1 antibody, anti GPD antibody, anti CTA-134P22.3 antibody, anti CD234 antibody, anti FY antibody, anti OTTHUMP00000060081 antibody, anti Duffy blood group, chemokine receptor antibody, anti Duffy blood group antigen antibody, anti Glycoprotein D antibody, anti Dfy antibody, anti GpFy antibody, anti WBCQ1 antibody, anti Fy glycoprotein antibody, anti OTTHUMP00000035293 antibody, anti OTTHUMP00000035294 antibody

Western blot analysis of human liver lysate using DARC antibody

Immunohistochemical staining of human kidney using DARC antibody

Immunohistochemical staining of Human Lung using DARC antibody

Quick Database Links

Gene Symbol

Duffy / FY / DARC

NCBI Gene ID

2532