CRISP2 (aa77-89) Antibody

SKU: orb20488

Description

Goat polyclonal to CRISP2

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:128000, WB: 0.05-0.1 μg/ml
• Predicted Reactivity: Bovine, Canine, Human, Porcine
• Application Notes:
  Reported variants represent identical protein: NP_003287.1, NP_001135879.1, NP_001135889.1, NP_001135907.1, NP_001135880.1.ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: In transfected HEK293 transiently expressing Human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CRISP2 (aa77-89)
• Protein Sequence: KCTLQHSDPEDRK
• Molecular Weight: 27.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CRISP2 antibody, anti cysteine-rich secretory protein 2 antibody, anti CRISP-2 antibody, anti GAPDL5 antibody, anti MGC111136 antibody, anti TPX1 antibody, anti TSP1 antibody, anti OTTHUMP00000016589 antibody, anti glyceraldehyde-3-phosphate dehydrogenase-like 5 antibody, anti testis specific protein 1 (probe H4-1 p3-1) antibody

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with orb20488 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with orb20468 (1mg/ml) in La

Quick Database Links

Gene Symbol

CRISP2 (aa77-89)

NCBI Gene ID

7180