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Catalog Number | orb19034 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to COMT |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, WB |
Reactivity | Canine, Human |
Dilution range | ELISA: 1:8000, WB: 0.3-1 μg/ml |
Conjugation | Unconjugated |
MW | 30.0; 24.5 |
Target | COMT (N Terminus) |
Entrez | 1312 |
Protein Sequence | GDTKEQRILNHVLQC |
RRID | AB_10755751 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti COMT antibody, anti catechol-O-methyltransfer Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 26kDa plus 29kDa bands observed in Human Testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and of 24.5kDa according to NP_009294.1). Recommended concentration: 0.3-1 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Staining of human testis lysate (35ug protein in RIPA buffer) with orb19034 (0.3ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.