CHRNB3 Antibody

SKU: orb19908

Description

Goat polyclonal antibody to CHRNB3

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:16000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 50kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 52.7kDa according to NP_000740.1). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CHRNB3
• Protein Sequence: SRHVKKEHFISQ
• Molecular Weight: 52.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CHRNB3 antibody, anti cholinergic receptor, nicotinic, beta 3 antibody, anti acetylcholine receptor, neuronal nicotinic, beta-3 subunit antibody, anti cholinergic receptor, nicotinic, beta polypeptide 3 antibody

orb19908 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

CHRNB3

NCBI Gene ID

1142