CHMP5 Antibody

• Catalog Number: orb18993
• Category: Antibodies
• Description: Goat polyclonal antibody to CHMP5
• Target: CHMP5
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: NKDGVLVDEFGLPQ
• RRID: AB_10755743
• MW: 24.6
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 28kDa band observed in lysates of K562 (calculated MW of 24.5kDa according to NP_057494.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ward et al, J Biol Chem. 2005 Mar 18;280(11): 10548-55.; PMID: 15644320). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti CHMP5 antibody, anti chromatin modifying prot
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 51510

orb18993 (xug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.