CHMP5 Antibody

SKU: orb18993

Description

Goat polyclonal antibody to CHMP5

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:8000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 28kDa band observed in lysates of K562 (calculated MW of 24.5kDa according to NP_057494.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ward et al, J Biol Chem. 2005 Mar 18;280(11): 10548-55.; PMID: 15644320). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CHMP5
• Protein Sequence: NKDGVLVDEFGLPQ
• Molecular Weight: 24.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CHMP5 antibody, anti chromatin modifying protein 5 antibody, anti HGNC:26942 antibody, anti C9orf83 antibody, anti CGI-34 antibody, anti HSPC177 antibody, anti PNAS-2 antibody, anti SNF7DC2 antibody, anti SNF7 domain containing 2 antibody, anti chromosome 9 open reading frame 83 antibody, anti Vps60 antibody

orb18993 (xug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

CHMP5

NCBI Gene ID

51510