CHCHD3 Antibody

SKU: orb20494

Description

Goat polyclonal antibody to CHCHD3

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat, Sheep
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 26kDa band observed in Human Brain (Cerebellum) and Heart lysates (calculated MW of 26.2kDa according to NP_060282.1). Recommended concentration: 1-3 μg/ml.Anonymous customer finds ~30kDa in THP1 and in human primary monocyte derived dendritic cells.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CHCHD3
• Molecular Weight: 26.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti coiled-coil-helix-coiled-coil-helix domain containing 3 antibody, anti FLJ20420 antibody, anti CHCHD3 antibody

orb20494 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Heart using CHCHD3 antibody

Immunohistochemical staining of Human Skeletal Muscle using CHCHD3 antibody

Quick Database Links

Gene Symbol

CHCHD3

NCBI Gene ID

54927