Centromere protein F/mitosin Antibody

SKU: orb18953

Description

Goat polyclonal to Centromere protein F

Research Area

Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:64000, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 358kDa according to NP_057427. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Centromere protein F / mitosin
• Protein Sequence: GLESKGSENCKVQ
• Molecular Weight: 358
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CENPF antibody, anti centromere protein F (350/400kD, mitosin) antibody, anti CENF antibody, anti PRO1779 antibody, anti mitosin antibody, anti AH antigen antibody, anti CENP-F kinetochore protein antibody, anti centromere protein F (400kD) antibody, anti cell-cycle-dependent 350K nuclear protein antibody, anti centromere protein F, 350/400ka (mitosin) antibody, anti RP11-262H5.1 antibody, anti hcp-1 antibody, anti centromere protein F antibody, anti mitosin antibody

Quick Database Links

Gene Symbol

Centromere protein F / mitosin

NCBI Gene ID

1063