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Goat anti-CD44 Antibody

SKU: orb146473

Description

Goat polyclonal to CD44

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:128000, WB: 0.01-0.03 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman
Application Notes
Approx 75-82kDa band observed in lysates of cell line U-2 OS (calculated MW of 81.5kDa according to NP_000601.3 and 76.6 according to NP_001001389.1). Recommended concentration: 0.01-0.03 μg/ml.Experiment Notes: This antibody is expected to recognize reported isoforms 1, 2, 3, 4, 6, 7, 8 (NP_000601.3; NP_001001389.1; NP_001001390.1; NP_001001391.1; NP_001189484.1; NP_001189485.1; NP_001189486.1).

Key Properties

ClonalityPolyclonal
TargetCD44
Protein SequenceNRDGTRYVQKGEYRT
Molecular Weight81.5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CD44, CD44 molecule (Indian blood group), CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CD44 antigen, GP90 lymphocyte homing/adhesion receptor, Hermes antigen, cell surface glycoprotein CD44, chondroitin sulfate proteoglyc
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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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