ASF1A Antibody

• Catalog Number: orb19690
• Category: Antibodies
• Description: Goat polyclonal antibody to ASF1A
• Target: ASF1A
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: ENSLNVMLESH
• RRID: AB_10753573
• MW: 23
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 21kDa band observed in lysates of cell line Jurkat (calculated MW of 23.0kDa according to NP_054753.1). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ASF1A antibody, anti ASF1 anti-silencing func
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 25842

orb19690 (1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.