Goat anti-AOC3 Antibody

• Catalog Number: orb19410
• Category: Antibodies
• Description: Goat polyclonal antibody to AOC3
• Target: AOC3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DEDPSFYSADSIY
• RRID: AB_10752575
• MW: 84.6
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:64000, WB: 0.01 μg/ml, IHC-P: 3-5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Liver shows membranous staining in sinusoid lining cells surrounding hepatocytes. Recommended concentration, 3-5ug/ml.WB: Preliminary experiments gave an approx 55kDa band in Human Adipose and Lung lysates after 0.01 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 84.6kDa according to NP_003725.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti AOC3 antibody, anti amine oxidase, copper con
• Note: For research use only
• Entrez: 8639

orb19410 (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.