Goat anti-AMACR (aa312-326) Antibody

• Catalog Number: orb195339
• Category: Antibodies
• Description: Goat polyclonal antibody to AMACR
• Target: AMACR (aa312-326)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: RGSFITSEEQDVSPR
• MW: 42.4; 22.2; 43.9
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Application notes: WB: Approx 40-42kDa band observed in Human Kidney, Liver and Coloretal cancer lysates, and in lysates of cell line CaCo-2, and approx 40.45kDa band observed in lysates of cell line LNCaP (calculated MW of 42.4kDa according to NP_055139.4) and 43.9kDa accordi. IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti AMACR antibody, anti alpha-methylacyl-CoA rac
• Note: For research use only
• Entrez: 23600

Immunohistochemical staining of Human Kidney using AMACR antibody

Western blot analysis of CaCo-2 (A) and LNCaP (B) cell lysate using AMACR antibody

Western blot analysis of Human Kidney (A), Liver (B) and Colorectal cancer (C) lysate using AMACR antibody