ALMS1 Antibody

SKU: orb18489

Description

Goat polyclonal antibody to ALMS1

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IF
• Dilution Range: ELISA: 1:4000, IF/ICC: 10μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis shows staining of cytoplasm in spermatocytes. Recommended concentration, 1-3ug/ml.WB: Preliminary experiments gave bands at approx 200kDa and 75kDa in lysates of Human T cell line Jurkat after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 461kDa according to NP_055935.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ALMS1
• Protein Sequence: RVTNQLLGRKVPWD
• Molecular Weight: 461
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ALMS1 antibody, anti Alstrom syndrome 1 antibody, anti ALSS antibody, anti KIAA0328 antibody, anti DKFZp686A118 antibody, anti DKFZp686D1828 antibody

Immunohistochemical staining of Human Testis using ALMS1 antibody.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using ALMS1 antibody.

Immunofluorescence analysis of HepG2 cells using ALMS1 antibody.

Quick Database Links

Gene Symbol

ALMS1

NCBI Gene ID

7840