ALMS1 Antibody

• Catalog Number: orb18489
• Category: Antibodies
• Description: Goat polyclonal antibody to ALMS1
• Target: ALMS1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: RVTNQLLGRKVPWD
• RRID: AB_10754459
• MW: 461
• Tested applications: ELISA, IF
• Dilution range: ELISA: 1:4000, IF/ICC: 10μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis shows staining of cytoplasm in spermatocytes. Recommended concentration, 1-3ug/ml.WB: Preliminary experiments gave bands at approx 200kDa and 75kDa in lysates of Human T cell line Jurkat after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 461kDa according to NP_055935.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ALMS1 antibody, anti Alstrom syndrome 1 antib
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 7840

Immunohistochemical staining of Human Testis using ALMS1 antibody.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using ALMS1 antibody.

Immunofluorescence analysis of HepG2 cells using ALMS1 antibody.