Goat anti-AIRE Antibody

SKU: orb18719

Description

Goat polyclonal antibody to AIRE

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 1-2 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration, 2.5ug/ml.WB: Appr. 60kDa in transfected HEK293 lysates (calculated MW of 57.7kDa according to NP_000374.1). Recommended concentration: 0.01-0.03 μg/ml.Experiment Notes: ChIP: A customer has reported this antibody was successful in ChIP (no data).

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: AIRE
• Molecular Weight: 57.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti AIRE antibody, anti autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy) antibody, anti APS1 antibody, anti APSI antibody, anti PGA1 antibody, anti APECED antibody, anti AIRE protein antibody, anti autoimmune regulator (APECED protein) antibody, anti autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) antibody, anti autoimmune regulator antibody, anti AIRE1 antibody, anti OTTHUMP00000109529 antibody, anti autoimmune polyendocrinopathy candidiasis ectodermal dystrophy antibody

Immunohistochemical staining of Human Thymus using AIRE antibody

Immunohistochemical staining of Human Thymus using AIRE antibody

Western blot analysis of Human Lymph Node lysate using AIRE antibody

WB analysis of Jurkat cell (A), Human Spleen (B) and Human Smooth Muscle (C) lysate protein using AIRE antibody

Immunofluorescence analysis of U2OS cells using AIRE antibody

Immunofluorescence analysis of HeLa cells using AIRE antibody

Flow cytometric analysis of Jurkat cells using AIRE antibody

Quick Database Links

Gene Symbol

AIRE

NCBI Gene ID

326