Goat anti-Actin-like 7B (mouse) Antibody

• Catalog Number: orb19439
• Category: Antibodies
• Description: Goat polyclonal antibody to Actl7b
• Target: Actin-like 7B (mouse)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Protein Sequence: DELHVDYELPDGK
• RRID: AB_10746658
• MW: 45.6
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 0.5-1.5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 45kDa band observed in Rat Testis lysates (calculated MW of 45.5kDa according to Rat NP_001020588.1 and 45.6kDa according to Mouse NP_079547.1). Recommended concentration: 0.5-1.5 μg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ACTL7B antibody, anti MGC151192, MGC151194, T
• Note: For research use only

orb19439 (0.5ug/ml) staining of Rat Testes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.