ACOX2 Antibody

SKU: orb19454

Description

Goat polyclonal antibody to ACOX2

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 75kDa band observed in Human Liver lysates (calculated MW of 76.8kDa according to NP_003491.1). Recommended concentration: 0.1-0.3 μg/ml. An additional band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ACOX2
• Protein Sequence: HQSRLRPSDPEAK
• Molecular Weight: 76.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ACOX2 antibody, anti acyl-Coenzyme A oxidase 2, branched chain antibody, anti BCOX antibody, anti BRCACOX antibody, anti BRCOX antibody, anti THCCox antibody, anti Peroxisomal branched chain acyl-CoA oxidase antibody, anti THCA-CoA oxidase antibody, anti Trihydroxycoprostanoyl-CoA oxidase antibody

orb19454 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

ACOX2

NCBI Gene ID

8309