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Catalog Number | orb1231282 |
---|---|
Category | Proteins |
Description | GM-CSF R alpha Recombinant Protein |
Tested applications | WB |
Tag | His Tag |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by SDS-PAGE. |
MW | 36.4 kDa |
Target | CSF2RA |
UniProt ID | P15509 |
Protein Sequence | Glu 23 - Gly 320 |
Source | HEK293 cells |
NCBI | NP_006131.2 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.4 |
Alternative names | GM-CSF R alpha,GM-CSF-R-alpha,GMCSFR-alpha,GMR-alp Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 36.4 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Human GM-CSF R alpha, His Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Unconjugated | |
95% | |
60.9 kDa | |
Human GM-CSF R alpha, Fc Tag (orb545735) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 320 (Accession # P15509-1). |
Unconjugated | |
95% | |
36.4 kDa | |
Human GM-CSF R alpha, His Tag (orb545734) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 320 (Accession # P15509-1). |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 60.6 kDa after removal of the signal peptide.The apparent molecular mass of GM-CSFR-hFc is approximately 70-130 kDa due to glycosylation. | |
Mammalian |
ELISA, WB | |
> 95% as determined by SDS-PAGE. | |
60.9 kDa | |
HEK293 cells |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result. |