GM-CSF mRNA-LNP (v2)

• Catalog Number: orb2719951
• Category: Proteins
• Description: GM-CSF (Granulocyte-macrophage colony-stimulating factor), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The GM-CSF receptor consists of an α chain and a β chain, which bind GM-CSF with low and high affinity, respectively, and the β chain is shared with the IL-3 and IL-5 receptors. The GM-CSF receptor (CSF2R) is present on myeloid cells and some non-hematopoietic cells, but is not expressed by lymphocytes such as T cells. Through CSF2R, GM-CSF triggers multiple signaling pathways, including phosphatidylinositol 3-kinase (PI3K), JAK/STAT, and ERK1/2 signaling. Due to the important role of GM-CSF as a colony-stimulating factor, GM-CSF has been used clinically to restore the bone marrow cell population of leukemia patients after chemotherapy/radiotherapy. In addition, GM-CSF is also involved in innate and adaptive immunity. Targeting GM-CSF may represent a novel approach to control adverse immune responses in autoimmune diseases and chronic inflammation. This product is designed as a tool for the delivery and expression of human GM-CSF2 mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of hGM-CSF2 mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The GM-CSF2 protein is about 16 kD and consists of 144 amino acids. The GenPept accession number is NP_000749.
• Form/Appearance: mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
• Application notes: Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.
• Storage: 4°C; ice
• Note: For research use only
• Expiration Date: 6 months from date of receipt.