Glypican 3 Rabbit Polyclonal Antibody

• Catalog Number: orb500683
• Category: Antibodies
• Description: Glypican 3 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human glypican 3 (501-580/580aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 65 kDa
• Target: GPC3
• UniProt ID: P51654
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: GPC3; glypican3; glypican-3; Intestinal protein OC
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-glypican 3 (orb500683) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.

Sample: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-glypican 3 (orb500683) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.

Sample: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-glypican 3 (orb500683) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.