Glypican 3 Antibody / GPC3

• Catalog Number: orb385791
• Category: Antibodies
• Description: Glypican-3 is a proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. [UniProt]
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Immunogen: Recombinant full-length human GPC3 protein was used as the immunogen for the Glypican-3 antibody.
• UniProt ID: P51654
• Tested applications: FACS, IF, IHC-P
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
• Application notes: Optimal dilution of the Glypican-3 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: SGPN3-1
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Glypican-3 antibody is available for research use only.
• Note: For research use only

Immunofluorescent staining of methanol-fixed HepG2 cells with Glypican-3 antibody (green, clone SGPN3-1) and Reddot nuclear stain (red).

IHC testing of FFPE human hepatocellular carcinoma with Glypican-3 antibody (clone SGPN3-1).

IHC testing of FFPE rat lung with Glypican-3 antibody (clone SGPN3-1).

Flow cytometry testing of PFA-fixed human HepG2 cells with Glypican-3 antibody (clone SGPN3-1); Red = isotype control, Blue = Glypican-3 antibody.