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Catalog Number | orb385791 |
---|---|
Category | Antibodies |
Description | Glypican-3 is a proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. [UniProt] |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | SGPN3-1 |
Tested applications | FACS, IF, IHC-P |
Reactivity | Human, Rat |
Isotype | Mouse IgG1, kappa |
Immunogen | Recombinant full-length human GPC3 protein was used as the immunogen for the Glypican-3 antibody. |
Dilution range | Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2) |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This Glypican-3 antibody is available for research use only. |
UniProt ID | P51654 |
Storage | Store the Glypican-3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the Glypican-3 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of Human hepatocellular carcinoma using Glypican-3 antibody
Immunohistochemical staining of Rat lung using Glypican-3 antibody
FACS, IF, IHC-P | |
Human, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
FACS, IF, IHC-P | |
Human | |
Mouse | |
Recombinant | |
Unconjugated |
FACS, IF, IHC-P | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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