Glypican 3 Antibody / GPC3

• Catalog Number: orb1716249
• Category: Antibodies
• Description: Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm s tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilm s tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GPC3/7107
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 1-200 from the human GPC3 protein was used as the immunogen for the Glypican 3 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Glypican 3 antibody is available for research use only.
• UniProt ID: P51654
• Storage: Aliquot the Glypican 3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the Glypican 3 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.