Glycophorin A Antibody

• Catalog Number: orb386067
• Category: Antibodies
• Description: Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: JC 159
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Formalin fixed membrane from a Hairy cell leukemia was used as the immunogen for the Glycophorin A antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Glycophorin A antibody is available for research use only.
• UniProt ID: P02724
• Storage: Store the Glycophorin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Glycophorin A antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human placenta with Glycophorin A antibody (clone JC 159). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free Glycophorin A Antibody (clone JC 159). Confirmation of Integrity and Purity of the Antibody.

IHC testing of FFPE human tonsil tissue with Glycophorin A antibody (clone JC 159). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.