GPD1 Antibody (HRP)

SKU: orb344249

Description

Glycerol-3-Phosphate Dehydrogenase antibody (Peroxidase)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,500
• Reactivity: Rabbit
• Application Notes:
  Anti-Glycerol-3-Phosphate Dehydrogenase has been tested by western blot and is suitable to be assayed against 1.0 ug of Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle]
• Target: GPD1
• Purity: Glycerol-3-Phosphate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle]. Cross reactivity against Glycerol-3-Phosphate-Dehydrogenase from other sources is unknown.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Glycerol-3-Phosphate Dehydrogenase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glycerol-3-Phosphate Dehydrogenase Antibody, FLJ26652 antibody, G3PD antibody, Gdc-1 antibody, Glycerphosphate dehydrogenase antibody, GPD-C antibody, Gpd1 protein antibody

Western Blot results of Goat Anti-Glycerol 3 Phosphate-Dehydrogenase Peroxidase Conjugated. Lane 1: Opal Prestained Molecular weight Ladder. Lane 2: Glycerol 3 Phosphate-Dehydrogenase. Load: 1 µg. Primary Antibody: Goat anti-Glycerol 3 Phosphate-Dehydrogenase Peroxidase Conjugated Antibody at 1 µg/ml overnight at 4°C. Secondary Antibody: Donkey Anti-Goat HRP (p/n orb347032) at 1:40000 for 30 min at RT. Blocking: BlockOut (p/n orb348644) for 30 min at RT. Expect: ~37kDa.

Quick Database Links

Gene Symbol

GPD1

UniProt

P08507

RefSeq:3043365