Glutamine synthetase Rabbit Polyclonal Antibody

• Catalog Number: orb4662
• Category: Antibodies
• Description: Glutamine synthetase Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Human, Porcine, Sheep
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Glutamine synthetase (285-373/373aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 42 kDa
• Target: GLUL
• UniProt ID: P15104
• RRID: AB_10923461
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: GLUL; cell proliferation-inducing protein 59; GLNA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Yamada, Hiroshi Y. et al. Tumor-promoting/progressing role of additional chromosome instability in hepatic carcinogenesis in Sgo1 (Shugoshin 1) haploinsufficient mice Carcinogenesis, 36, 429-440 (2015)

PubMed: 25740822

Sample: Raji Cell (Human) Lysate at 40 ug, Primary: Anti-Glutamine synthetase (orb4662) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD.

Tissue/Cell: mouse brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Glutamine synthetase Polyclonal Antibody, Unconjugated (orb4662) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.