Cart summary

You have no items in your shopping cart.

    Glutamine Synthetase antibody (Biotin)

    Catalog Number: orb344322

    DispatchUsually dispatched within 5-10 working days
    $ 753.00
    Catalog Numberorb344322
    CategoryAntibodies
    DescriptionGlutamine Synthetase antibody (Biotin)
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsELISA, IP, WB
    ReactivityBacteria
    IsotypeIgG
    ImmunogenGlutamine Synthetase [Microbial]
    Concentration1.0 mg/mL
    Dilution rangeELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:2,500
    Form/AppearanceLyophilized
    PurityAnti-Glutamine Synthetase (microbial) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial]. Cross reactivity against Glutamine Synthetase from other sources is unknown.
    ConjugationBiotin
    UniProt IDQ79VE3
    NCBICAG33988.1
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesgoat anti-Glutamine Synthetase Antibody biotin Con
    Read more...
    NoteFor research use only
    Application notesAnti-Glutamine Synthetase (microbial) antibody has been assayed against 1.0 µg of Glutamine Synthetase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
    Expiration Date12 months from date of receipt.
    Glutamine Synthetase antibody (Biotin)

    Biorbyt Goat anti Glutamine Synthetase antibody was used to detect Glutamine Synthetase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in orb348637). Detection shown was using Dylight 649 conjugated Donkey anti goat (1:10K in TBS/orb348637) 1 hr RT.

    • GLUL antibody (Biotin) [orb41840]

      ELISA

      Human

      Rabbit

      Polyclonal

      Biotin

      100 μg, 50 μg
    • ASNS antibody (Biotin) [orb52377]

      ELISA

      Human

      Rabbit

      Polyclonal

      Biotin

      100 μg, 50 μg
    • Glutamine Synthetase antibody (Biotin) [orb344323]

      ELISA,  IP,  WB

      Bacteria

      Goat

      Polyclonal

      Biotin

      25 μl
    • Asparagine Synthetase antibody (Biotin) [orb453282]

      ELISA,  ICC,  IHC-Fr,  IHC-P,  WB

      Bovine, Canine, Equine, Porcine, Rabbit

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Biotin

      100 μl
    Submit a review

    Filter by Rating

      • Star
      • Star
      • Star
      • Star
      • Star
      • 5 stars
      • Star
      • Star
      • Star
      • Star
      • Star
      • 4 stars
      • Star
      • Star
      • Star
      • Star
      • Star
      • 3 stars
      • Star
      • Star
      • Star
      • Star
      • Star
      • 2 stars
      • Star
      • Star
      • Star
      • Star
      • Star
      • 1 stars