Glutamine Synthetase Antibody

• Catalog Number: orb344214
• Category: Antibodies
• Description: Glutamine Synthetase antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: Glutamine Synthetase [native from brevibacterium species]
• Concentration: 1.0mg/mL
• Dilution range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Anti-Glutamine Synthetase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial]. Cross reactivity against Glutamine Synthetase from other sources is unknown.
• Conjugation: Unconjugated
• UniProt ID: Q79VE3
• NCBI: CAG33988.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Glutamine Synthetase Antibody, Glutamine
• Note: For research use only
• Application notes: Anti-Glutamine Synthetase antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of Glutamine Synthetase [Microbial] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product. It is suitable for Immunohistochemistry also. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western Blot of Goat anti-Glutamine Synthetase antibody. Lane 1: Glutamine Synthetase Reduced. Lane 2: Glutamine Synthetase Non-Reduced. Load: ~1 ug of protein per lane. Primary antibody: Glutamine Synthetase antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 649 conjugated Donkey anti goat (1:10K in TBS/orb348637) 1 hr RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 53.3 kDa, 56 kDa for Glutamine Synthetase. Other band(s): Glutamine Synthetase splice variants and isoforms.

Western blot of Goat Anti-Glutamine Synthetase primary antibody. Lane 1: Glutamine Synthetase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Glutamine Synthetase antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase goat secondary antibody at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT. Observed/Predicted size: 54 kDa, 54 kDa for Glutamine Synthetase (Brevibacterium). Other band(s): None.