GLUT6 Rabbit Polyclonal Antibody

• Catalog Number: orb157189
• Category: Antibodies
• Description: GLUT6 Rabbit Polyclonal Antibody
• Target: SLC2A6
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human GLUT6 (201-300/507aa)
• UniProt ID: Q9UGQ3
• MW: 55 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Glucose Transporter GLUT6; A330096C23; F630103L12R
• Research Area: Cancer Research
• Note: For research use only

References

Zeng, Zhongzhen et al. Ginsenoside Rg3 inhibits angiogenesis in gastric precancerous lesions through downregulation of Glut1 and Glut4 Biomed Pharmacother, 145, 112086 (2022)

PubMed: 34799220

Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-GLUT6'GLUT9 (orb157189) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55kD, Observed band size: 55kD.

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-GLUT6'GLUT9 (orb157189) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55kD, Observed band size: 55kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GLUT6 Polyclonal Antibody, Unconjugated (orb157189) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GLUT6 Polyclonal Antibody, Unconjugated (orb157189) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.