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| Catalog Number | orb157189 |
|---|---|
| Category | Antibodies |
| Description | GLUT6 Rabbit Polyclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Human |
| Reactivity | Mouse, Rat |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human GLUT6 (201-300/507aa) |
| Antibody Type | Primary Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 55 kDa |
| Target | SLC2A6 |
| UniProt ID | Q9UGQ3 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | Glucose Transporter GLUT6; A330096C23; F630103L12R Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Zeng, Zhongzhen et al. Ginsenoside Rg3 inhibits angiogenesis in gastric precancerous lesions through downregulation of Glut1 and Glut4 Biomed Pharmacother, 145, 112086 (2022)
Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-GLUT6'GLUT9 (orb157189) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55kD, Observed band size: 55kD.
Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-GLUT6'GLUT9 (orb157189) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55kD, Observed band size: 55kD.
Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GLUT6 Polyclonal Antibody, Unconjugated (orb157189) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: rat spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GLUT6 Polyclonal Antibody, Unconjugated (orb157189) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
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