GLUT4 Rabbit Polyclonal Antibody

• Catalog Number: orb10728
• Category: Antibodies
• Description: GLUT4 Rabbit Polyclonal Antibody
• Target: SLC2A4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human GLUT4 (401-509/509aa)
• UniProt ID: P14672
• RRID: AB_10749205
• MW: 54 kDa
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: insulin-responsive; Glucose transporter GLUT 4; Gl
• Note: For research use only

Blank control (blue line): K562 (blue). Primary Antibody (green line): Rabbit Anti-GLUT4 antibody (orb10728), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 70% ethanol (overnight at 4°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-GLUT4 (orb10728) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.

Sample: Heart (Rat) Cell Lysate at 40 ug, Primary: Anti-GLUT4 (orb10728) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.

Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 2: Heart (Mouse) Lysate at 40 ug, Lane 3: Heart (Rat) Lysate at 40 ug, Lane 4: Muscle (Mouse) Lysate at 40 ug, Lane 5: Muscle (Rat) Lysate at 40 ug, Primary: Anti-GLUT4 (orb10728) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD.

Tissue/Cell: NIH/3T3 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (GLUT4) polyclonal Antibody, Unconjugated (orb10728) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated (orb10728) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.