GLUT3 Rabbit Polyclonal Antibody

SKU: orb10727

Description

GLUT3 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GLUT3 (151-260/493aa)
• Target: SLC2A3
• Molecular Weight: 54 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

facilitated glucose transporter member 3; Glucose Transporter GLUT3; FLJ90380; Glucose Transporter Type 3; Glucose transporter type 3 brain; GLUT 3; GLUT-3; GTR3_HUMAN; SLC2A3; Solute Carrier Family 2(Facilitated Glucose Transporter) Member 3; Solute carrier family 2.

Sample: 293T (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, Primary: at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 63 kD.

Sample: Brain (Mouse) Lysate at 40 ug, Brain (Rat) Lysate at 40 ug, 293T (Human) Cell Lysate at 30 ug, NIH/3T3 (Mouse) CellLysate at 30 ug, Primary: Anti-GLUT3 (orb10727) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 50/63 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Glut3 Polyclonal Antibody, Unconjugated (orb10727) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

SLC2A3

UniProt

P11169

References

Jones, Carolyn J. P. et al. Functional changes in Hofbauer cell glycobiology during human pregnancy Placenta, 36, 1130-1137 (2015)

PubMed: 26264896

Applications

IHC

Reactivity

Human