GLUT1 Antibody / SLC2A1

• Catalog Number: orb1824538
• Category: Antibodies
• Description: Recognizes a protein of 55kDa, which is identified as GLUT-1. Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are many types of glucose transport carrier proteins, designated as Glut-1 to Glut-12. Glut-1 is a major glucose transporter in the mammalian blood-brain barrier. It is expressed in high density on the membranes of human erythrocytes and the brain capillaries that comprise the blood-brain barrier. Glut-1 is expressed at variable levels in many human tissues. Overexpression of Glut-1 has been linked to tumor progression or poor survival of patients with carcinomas of the colon, breast, cervical, lung, bladder and mesothelioma. Glut-1 is a sensitive and specific marker for the differentiation of malignant mesothelioma (positive) from reactive mesothelium (negative).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GLUT1/7308
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: A recombinant fragment of human GLUT1 protein (within amino acids 200-400) was used as the immunogen for the SLC2A1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This SLC2A1 antibody is available for research use only.
• UniProt ID: P11166
• Storage: Aliquot the SLC2A1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human esophagus tissue with SLC2A1 antibody (clone GLUT1/7308). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human ovarian carcinoma tissue with SLC2A1 antibody (clone GLUT1/7308). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free SLC2A1 antibody (clone GLUT1/7308) as confirmation of integrity and purity.