GLUR2 Rabbit Polyclonal Antibody

SKU: orb13619

Description

GLUR2 Rabbit Polyclonal Antibody

Research Area

Glutamate, Glutamate Receptors, Neuroscience

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GluR2 (151-250/883aa)
• Target: GRIA2
• Molecular Weight: 117 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GLUR2; GLURB; GluA2; GluR-K2; HBGR2; NEDLIB; gluR-2; gluR-B; GRIA2_HUMAN; GRIA2; AMPA-selective glutamate receptor 2; Glutamate receptor ionotropic, AMPA 2; GRIA2_MOUSE; GRIA2_RAT;

Blank control: RSC96 (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-AF647 (white blue), Dilution: 1:100 in 1X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 ugl in 100 uL 1X PBS containing 0.5% BSA (green).

Blank control: RSC96 (blue), Isotype Control Antibody: Rabbit IgG-AF647 (orange), Primary Antibody Dilution: 5 uL in 100 uL 1X PBS containing 0.5% BSA (green).

Blank control: RSC96 (blue), Isotype Control Antibody: Rabbit IgG-AF647 (orange), Primary Antibody Dilution: 5 uL in 100 uL 1X PBS containing 0.5% BSA (green).

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GLUR2) Polyclonal Antibody, Unconjugated (orb13619) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GLUR2) Polyclonal Antibody, Unconjugated (orb13619) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: HepG2 Cell Lysate at 30 ug, Primary: Anti-GluR2 (orb13619) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 97 kD, Observed band size: 117 kD.

Tissue/Cell: mouse brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GLUR2 Polyclonal Antibody, Unconjugated (orb13619) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

GRIA2

UniProt

P42262