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Catalog Number | orb196279 |
---|---|
Category | Antibodies |
Description | Glucose Transporter GLUT4/SLC2A4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human GLUT4 recombinant protein (Position: N333-D509). Human GLUT4 shares 97% amino acid (aa) sequence identity with mouse GLUT4. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, -Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Rat, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 54787 MW |
UniProt ID | P14672 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Solute carrier family 2, facilitated glucose trans Read more... |
Note | For research use only |
Application notes | WB: The detection limit for GLUT4 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of GLUT4 using anti-GLUT4 antibody.Lane 1:rat cardiac muscle Tissue;2:rat skeletal muscle Tissue.
IHC analysis of GLUT4 using anti-GLUT4 antibody.GLUT4 was detected in frozen section of rat cardiac muscle tissues.
IHC analysis of GLUT4 using anti-GLUT4 antibody.GLUT4 was detected in frozen section of mouse cardiac muscle tissues.
IHC analysis of GLUT4 using anti-GLUT4 antibody.GLUT4 was detected in paraffin-embedded section of human intestinal cancer tissues.
IHC analysis of GLUT4 using anti-GLUT4 antibody.GLUT4 was detected in paraffin-embedded section of rat cardiac muscle tissues.
IHC analysis of GLUT4 using anti-GLUT4 antibody.GLUT4 was detected in paraffin-embedded section of mouse skeletal muscle tissues.
IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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