Glucose Transporter GLUT1/SLC2A1 Antibody

• Catalog Number: orb259612
• Category: Antibodies
• Description: Glucose Transporter GLUT1/SLC2A1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human SLC2A1 recombinant protein (Position: R92-V492). Human SLC2A1 shares 98% and 98.3% amino acid (aa) sequence identity with mouse and rat SLC2A1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, - Immunocytochemistry/Immunofluorescence , 2μg/ml, Human, - Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 54084 MW
• UniProt ID: P11166
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Solute carrier family 2, facilitated glucose trans
• Note: For research use only
• Application notes: WB: The detection limit for SLC2A1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-SLC2A1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of SLC2A1 using anti-SLC2A1 antibody.Lane 1:rat brain Tissue;2:mouse brain Tissue;3:mouse NIH3T3 Cell.

WB analysis of SLC2A1 using anti-SLC2A1 antibody.Lane 1:human placenta tissue; 2:human A431 cell; 3:mouse brain tissue; 4:rat PC-12 cell; 5:mouse NIH/3T3 cell.

IF analysis of SLC2A1 using anti-SLC2A1 antibody. SLC2A1 was detected in immunocytochemical section of HepG2 cells.

IHC analysis of SLC2A1 using anti-SLC2A1 antibody.SLC2A1 was detected in paraffin-embedded section of Mouse Brain Tissue.

IHC analysis of SLC2A1 using anti-SLC2A1 antibody.SLC2A1 was detected in paraffin-embedded section of Rat Brain Tissue.

IHC analysis of SLC2A1 using anti-SLC2A1 antibody.SLC2A1 was detected in paraffin-embedded section of Human Placenta Tissue.

IHC analysis of SLC2A1 using anti-SLC2A1 antibody.SLC2A1 was detected in frozen section of Human Placenta Tissue.

IHC analysis of SLC2A1 using anti-SLC2A1 antibody.SLC2A1 was detected in immunocytochemical section of A549 Cell.

IHC analysis of SLC2A1 using anti-SLC2A1 antibody. SLC2A1 was detected in paraffin-embedded section of mouse brain tissue.

IHC analysis of SLC2A1 using anti-SLC2A1 antibody. SLC2A1 was detected in paraffin-embedded section of rat brain tissue.

IHC analysis of SLC2A1 using anti-SLC2A1 antibody. SLC2A1 was detected in paraffin-embedded section of rat brain tissue.

IHC analysis of SLC2A1 using anti-SLC2A1 antibody. SLC2A1 was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of SLC2A1 using anti-SLC2A1 antibody. SLC2A1 was detected in paraffin-embedded section of human placenta cancer tissue.

IHC analysis of SLC2A1 using anti-SLC2A1 antibody. SLC2A1 was detected in paraffin-embedded section of human placenta cancer tissue.