Glucose Dehydrogenase Antibody Biotin Conjugated

SKU: orb344307

Description

Glucose Dehydrogenase antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
• Reactivity: Bacteria
• Application Notes:
  Anti-Glucose Dehydrogenase has been assayed against 1.0 ug of Glucose Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Glucose Dehydrogenase [Bacillus species]
• Purity: Anti-Glucose Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glucose Dehydrogenase [Bacillus species]. Cross reactivity against Glucose Dehydrogenase from other sources is unknown.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

goat anti-Glucose Dehydrogenase Antibody biotin Conjugation, biotin Conjugated goat anti-Glucose Dehydrogenase Antibody, gdh antibody, gdh2 antibody, gdh3 antibody, gdh4 antibody, gdhI antibody, GLCDHIII antibody, GLCDHIV antibody, Glucose 1 dehydrogenase 1 antibody

Biorbyt Goat anti Glucose Dehydrogenase antibody (orb750402) was used to detect purified Glucose Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in orb348637). Detection shown was using Dylight 488 conjugated Donkey anti goat (1:10K in TBS/orb348637 1 hr RT).

Quick Database Links

UniProt

P07999

RefSeq:AAA22475.1