GLD2 Antibody (N-term)

• Catalog Number: orb1930671
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This GLD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human GLD2.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q6PIY7
• MW: 56028 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:25, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, IHC-P: 1:25
• Clone Number: RB26128
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Poly(A) RNA polymerase GLD2, hGLD-2, PAP-associate
• Note: For research use only
• NCBI: NP_001107865.1, NP_776158.2, NP_001107866.1

Immunohistochemical analysis of paraffin-embedded H. cerebellum section using GLD2 Antibody (N-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Fluorescent image of MCF-7 cells stained with GLD2 Antibody (N-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

GLD2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLD2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of lysates from mouse brain, mouse cerebellum tissue lysate (from left to right), using GLD2 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

Western blot analysis of lysates from HepG2 cell line and mouse brain tissue lysate (from left to right), using GLD2 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.