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Catalog Number | orb614118 |
---|---|
Category | Antibodies |
Description | GLB1/Beta-galactosidase Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived rat GLB1/Beta-galactosidase recombinant protein (Position: R103-I646). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.25-0.5μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Rat Flow Cytometry, 1-3μg/1x106 cells, Mouse, Rat Direct ELISA, 0.1-0.5μg/ml, Rat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 65 kDa, 76 kDa, 85 kDa |
UniProt ID | D3ZUM4 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Beta-galactosidase; Glb1; Glb1_mapped; rCG_25043 Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
IF analysis of GLB1 using anti-GLB1 antibody.
Flow Cytometry analysis of RH35 cells using anti-GLB1 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of HEPA 1-6 cells using anti-GLB1 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.
IHC analysis of GLB1 using anti-GLB1 antibody.
WB analysis using anti-GLB1 antibody.Lane 1:rat lung tissue, Lane 2:rat liver tissue, Lane 3:rat testis tissue, Lane 4:mouse liver tissue, Lane 5:mouse testis tissue, Lane 6:mouse HEPA1-6 cell, Lane 7:mouse Neuro-2a cell.
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, IHC-P, WB | |
Canine, Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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